PUC

Methods: The full length L 1 coden region of HPV 58 was amplified by PCR, and cloned into pUC 19, sequenced.

方法: 用聚合酶链反应(PCR)扩增HPV58L1完整编码区基因,将PCR扩增产物 克隆至pUC19质粒中并测序.

In the course of the investigation, the PUC charge related to the leadership of basaltic sub-command.

在侦查过程中, 市局相关领导亲自坐镇玄武 分局 指挥.

The recombinant plasmid pPGVT 3 derived from the vector pUC 4 was unstable in the E.

从载体质粒pUC4衍生的重组质粒pPGVT3在大肠杆菌宿主DF2145中是不稳定的,以pPGVT3转化DF2145时在4o℃ 培养得不到转化子.

PUC 18 transferring and ERIC-PCR showed that the recombination strain SR-15 could grow in the plant stably.

SR-15 菌株通过质粒PUC-18转化和ERIC -PCR 再分离实验验证,结果显示重组菌株在植株体内稳定定位,具有稳定的内生特性.

A recombinant plasmid pUC-IL 6 coding for IL 6 was constructed by recombinant gene technique.

利用基因重组技术,构建含IL6基因的重组质粒pUC - IL6.

Results: The recombinant plasmid pUC 18 E 6 had been got.

结果: 获得重组质粒pUC18E6.

The dnaA gene with LacZ promoter was cloned into pUC 19 using the Escherichia coli DH 5 a strain.

将带有LacZ启动 子的dnaA基因构建到pUC19中,得到重组质粒pUCA12.

E . coli DH 5α was transformed by pUC 18 recombined with PRV CP gene cDNA.

香木瓜环斑病毒(PRV) 外壳蛋白(CP)基因cDNA克隆 到pUC18上构建成 大肠杆菌 (Escherickiacoli)表达载体.

The insert cDNAs were clonedinto EcoR I-digested, BAP-treated pUC 18 vectors and transformed into E.

同时以EcoR酶消化环状pUC18DNA, BAP对载体5端去磷酸化.