According to the procedure a plasmid DNA& pBR 322 and pAt 153 was obtained. The frequency of transformation of genetic markers of resistant antibiotics has been determined using E. Coli C_ ( 600) and HB 101 as the recipient cell respectively.

  • 本文叙述了一种简便有效的纯化质粒DNA的方法,制得的质粒DNA&pBR322和pAt153分别以C600和HB101作为受体细胞进行转化,测定了对抗生素有抗性的遗传标记的转化频率。

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